@@ -51,6 +51,8 @@ The following figure summarizes the twelve pipelines compared in our study :
In our study, we analyze the results of a paired-end sequencing, after extraction and amplification of filtrated eDNA from aquarium seawater, to detect intraspecific haplotypic variability in *Mullus surmuletus*. Only one aquarium is given as example in the scripts.
The following scripts were used for the bioinformatics analysis of [this publication](https://doi.org/10.1002/edn3.269), based on the datasets available [here](https://zenodo.org/record/4570303).
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## II - Installation
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@@ -381,4 +383,4 @@ For more details on this VSEARCH chimera removal step, see the original UCHIME2
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## VII - Analyse your results
Now you can make a statistical analysis to evaluate your filtering quality, after comparing the amplicons returned by the pipeline with your reference dataset.
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Now you can make a statistical analysis to evaluate your filtering quality, after comparing the amplicons returned by the pipeline with your reference dataset.
