Commit cd981eb6 authored by peguerin's avatar peguerin
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parent eba8bb98
Only_obitools pipeline
# Table of contents
1. [Introduction](#1-introduction)
2. [Installation](#2-installation)
1. [Requirements](#21-requirements)
2. [Optional components](#22-optional-components)
3. [Reporting bugs](#3-reporting-bugs)
4. [Running the pipeline](#4-running-the-pipeline)
# 1. Introduction
Here, we reproduce the bioinformatics pipeline used by *SPYGEN* to generate species environmental presence from raw eDNA data. This pipeline is based on *OBItools* a set of python programs designed to analyse Next Generation Sequencer outputs (illumina) in the context of DNA Metabarcoding.
# 2. Installation
You will need to install
## 2.1. Requirements
In order to run "only_obitools", you need a couple of programs. Most of
them should be available pre-compiled for your distribution. The
programs and libraries you absolutely need are:
- [OBItools](
## 2.2. Optional components
Other libraries are optional and only limit the features that are
built. These include:
# 3. Reporting bugs
If you're sure you've found a bug — e.g. if one of my programs crashes
with an obscur error message, or if the resulting file is missing part
of the original data, then by all means submit a bug report.
I use [GitLab's issue system](
as my bug database. You can submit your bug reports there. Please be as
verbose as possible — e.g. include the command line, etc
# 4. Running the pipeline
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