# snakemake_rapidrun_swarm

OTU clustering with SWARM on RAPIDRUN data encapsulated in SNAKEMAKE


# Prerequisites


* python3

* snakemake

* singularity

python3 dependencies

```
pip3 install pandas
pip3 install biopython
```

python3 dependencies to run `snakemake`:

```
pip3 install datrie
pip3 install ConfigArgParse
pip3 install appdirs
pip3 install gitdb2
```

# Configuration / input files

You have to set 2 files:

* [01_infos/all_samples.tsv](01_infos/all_samples.tsv)
* [01_infos/config.yaml](01_infos/config_test.yaml)


# Run the workflow

```
CORES=32
CONFIGFILE="../01_infos/config_test.yaml"
bash main.sh $CORES $CONFIGFILE
```


# Run from scratch


## clone repositories

```
git clone git@gitlab.mbb.univ-montp2.fr:edna/snakemake_rapidrun_swarm.git
cd snakemake_rapidrun_swarm
```

## write demultiplexing table

From
- `01_infos/config_test.yaml` 
	* `fichiers:` `rapidrun`
	* `fichiers:` `dat`

:warning: the colon "marker" into `fichiers:` `rapidrun` must be the same name as marker's keys of `fichiers:` `dat` into `01_infos/config_test.yaml` 

This will generate a file `01_infos/all_demultiplex.csv` with each line arguments for command.

```
snakemake --configfile 01_infos/config_test.yaml -s readwrite_rapidrun_demultiplexing.py
```


## merge fastq

From
- `01_infos/config_test.yaml` 
	* `fichiers:` `rapidrun`

Deduce the `{run}` fastq paired-end to merge

:warning: guadeloupe have been removed by hand into 02_assembly/Snakefile !!!


```
cd 02_assembly
snakemake --configfile $CONFIGFILE -s Snakefile -j $CORES --use-singularity --singularity-args "--bind /media/superdisk:/media/superdisk" --latency-wait 20
cd ..
```

## demultiplexing

From
- `01_infos/config_test.yaml` 
	* `fichiers:` `rapidrun`

will generate demultiplexed .fasta and .qual files for each `{projet}`/`{marker}`/`{sample}` into `03_demultiplexing`


```
cd 03_demultiplexing
snakemake --configfile $CONFIGFILE -s Snakefile -j $CORES --use-singularity --singularity-args "--bind /media/superdisk:/media/superdisk" --latency-wait 20
cd ..
```

## cat qualities

From
- `01_infos/config_test.yaml` 
	* `fichiers:` `rapidrun`

Simply concatenate and format .qual files by `{projet}`/`{marker}`

```
cd 04_cat_quality
snakemake --configfile $CONFIGFILE -s Snakefile -j $CORES --use-singularity --singularity-args "--bind /media/superdisk:/media/superdisk" --latency-wait 20
cd ..
```

## clustering

```
cd 05_clustering
snakemake --configfile $CONFIGFILE -s Snakefile -j $CORES --use-singularity --singularity-args "--bind /media/superdisk:/media/superdisk" --latency-wait 20
cd ..

```