Commit 7834aaf5 authored by lbenestan's avatar lbenestan 💬
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Update README.md

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......@@ -43,12 +43,8 @@ We will be receive the baits in one unique tube.
# Hybridization baits and sequencing
Baits will be received as DNA but will be soon transformed in RNA (to facilitate the hybridization) based on the same step described in the hyRAD protocol.
Hybridation will allow **5 to 10% of divergence** between the DNA and the baits sequence, allowing 9 SNPs per sequence captured at the maximum.
Specificity of baits will be adjusted regarding the temperature of the hybridization.
The removal of non ***Rana temporaria*** sequences will be done by a post informatic treatment and by sequencing on Miseq our eDNA samples, to screen for other species present in the eDNA sample.
The baits will be received as DNA but will be rapidly transformed into RNA (to facilitate hybridization) following the same step described in the hyRAD protocol.
Hybridization will allow **5 to 10% divergence** between the DNA sequence and the bait sequence, allowing a maximum of 9 SNPs per sequence captured.
The specificity of the baits will be adjusted according to the hybridization temperature, which means that two hybridization-captures are usually performed, one at 55°C and the other at 65°C (for more specificity). No species phylogenetically related to Rana temporaria are present on the ponds we sampled.
Removal of non-Rana temporaria*** sequences will be done by post-computational processing and Miseq sequencing of our eDNA samples to screen for other species present in the eDNA sample.
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