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khalid
radsex
Commits
0a592601
Commit
0a592601
authored
Oct 29, 2018
by
RomainFeron
Browse files
Removed unused demultiplexing code
parent
449904fb
Changes
3
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Inline
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src/demultiplexing.cpp
deleted
100755 → 0
View file @
449904fb
#include "demultiplexing.h"
KSEQ_INIT
(
gzFile
,
gzread
)
// Kseq init macro
void
demultiplexing
(
Parameters
&
parameters
)
{
/* Demultiplexing is actually not a trivial task if done correctly.
* It may get implemented later but for now this pipeline relies on Stacks for this step.
*/
const
std
::
string
sbf1
=
"TGCA"
;
const
std
::
unordered_map
<
std
::
string
,
std
::
string
>
barcodes
=
load_barcodes
(
parameters
);
std
::
string
par
=
"output_dir_path"
;
std
::
string
output_dir_path
=
parameters
.
get_value_from_name
<
std
::
string
>
(
par
);
std
::
ofstream
log_file
(
output_dir_path
+
"demultiplexing.log"
);
std
::
ofstream
summary_file
(
output_dir_path
+
"summary.tsv"
);
std
::
unordered_map
<
std
::
string
,
uint64_t
>
statistics
;
std
::
unordered_map
<
std
::
string
,
gzFile
>
output_files
;
// Output files are mapped to their barcodes.
std
::
string
output_file_path
;
for
(
auto
b
:
barcodes
)
{
output_file_path
=
output_dir_path
+
b
.
second
+
".fq.gz"
;
// Create a compressed output file, the number after wb indicates the level of compression (1 -> low, 9 -> high)
output_files
[
b
.
first
]
=
gzopen
(
output_file_path
.
c_str
(),
"wb9"
);
if
(
!
output_files
[
b
.
first
])
std
::
cout
<<
std
::
endl
<<
"** Error: could not open output file
\"
"
<<
output_file_path
<<
"
\"
"
<<
std
::
endl
;
}
gzFile
input_file
;
kseq_t
*
sequence
;
// Create the seq object
int
line_n
;
// Stores the length of a line
std
::
string
revcomp
=
""
;
char
nuc
=
'A'
;
uint
count
;
par
=
"input_file_path"
;
input_file
=
gzopen
(
parameters
.
get_value_from_name
<
std
::
string
>
(
par
).
c_str
(),
"r"
);
sequence
=
kseq_init
(
input_file
);
// Initialize the seq object
// Read through the file and store the results
while
((
line_n
=
kseq_read
(
sequence
))
>=
0
)
{
rev_comp
(
sequence
->
seq
.
s
,
revcomp
,
nuc
);
++
count
;
}
kseq_destroy
(
sequence
);
// Destroy the seq object
gzclose
(
input_file
);
// Close all output files
for
(
auto
&
ofile
:
output_files
)
{
gzclose
(
ofile
.
second
);
}
}
src/demultiplexing.h
deleted
100755 → 0
View file @
449904fb
#pragma once
#include <string>
#include <vector>
#include <fstream>
#include <unordered_map>
#include <unordered_set>
#include <zlib.h>
#include "utils.h"
#include "parameters.h"
#include "barcodes_file.h"
#include "kseq/kseq.h"
// Main function implementing the analysis
void
demultiplexing
(
Parameters
&
parameters
);
src/radsex.h
View file @
0a592601
...
...
@@ -6,7 +6,6 @@
#include "process_reads.h"
#include "subset.h"
#include "frequencies.h"
#include "demultiplexing.h"
#include "group_loci.h"
#include "significant_sequences.h"
#include "mapping.h"
...
...
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