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mbb
waw
Commits
a4f95969
Commit
a4f95969
authored
May 19, 2022
by
khalid
Browse files
change WFs names
parent
78f95a87
Changes
1
Hide whitespace changes
Inline
Side-by-side
workflows/RnaSeqDemo/RnaSeqDemo.yaml
View file @
a4f95969
{
name
:
"
RnaSeqDemo"
,
docker_name
:
"
RnaSeqDemo"
,
description
:
"
RnaSeq
DE
d
emo"
,
description
:
"
RnaSeq
DE
D
emo"
,
version
:
"
0.0.1"
,
author
:
"
MBB"
,
options
:
[
...
...
@@ -14,72 +14,43 @@ options: [
},
],
steps_in
:
[
{
step_name
:
"
FROGS_2
"
,
tool_name
:
"
FROGS_preprocess"
,
rule_name
:
"
FROGS_preprocess_P
E"
,
{
step_name
:
"
quality
"
,
tool_name
:
"
trimmomatic"
,
rule_name
:
"
trimmomatic_S
E"
,
params
:
[
{
input_name
:
read
_dir
,
origin_command
:
raw_read
_dir
,
origin_name
:
read
_dir
,
origin_step
:
input
},
{
input_name
:
read
,
origin_command
:
raw_read
s
,
origin_name
:
read
,
origin_step
:
input
},
]
},
{
step_name
:
"
FROGS_4
"
,
tool_name
:
"
FROGS_cluster
"
,
rule_name
:
"
FROGS_cluster
"
,
{
step_name
:
"
quality_2
"
,
tool_name
:
"
fastqc
"
,
rule_name
:
"
fastqc_SE
"
,
params
:
[
{
input_name
:
sequence_file
,
origin_command
:
FROGS_preprocess_PE
,
origin_name
:
sequence_file
,
origin_step
:
FROGS_2
},
{
input_name
:
count_file
,
origin_command
:
FROGS_preprocess_PE
,
origin_name
:
count_file
,
origin_step
:
FROGS_2
},
{
input_name
:
read
,
origin_command
:
trimmomatic_SE
,
origin_name
:
read
,
origin_step
:
quality
},
]
},
{
step_name
:
"
FROGS
"
,
tool_name
:
"
FROGS_remove_chimera"
,
rule_name
:
"
FROGS_remove_chimera
"
,
{
step_name
:
"
mapping
"
,
tool_name
:
"
hisat2"
,
rule_name
:
"
hisat2_SE
"
,
params
:
[
{
input_name
:
sequence_file
,
origin_command
:
FROGS_cluster
,
origin_name
:
cluster_seeds
,
origin_step
:
FROGS_4
},
{
input_name
:
abundance_biom
,
origin_command
:
FROGS_cluster
,
origin_name
:
abundance_file
,
origin_step
:
FROGS_4
},
{
input_name
:
read
,
origin_command
:
trimmomatic_SE
,
origin_name
:
read
,
origin_step
:
quality
},
{
input_name
:
index
,
origin_command
:
raw_hisat2_index
,
origin_name
:
hisat2_index
,
origin_step
:
input
},
]
},
{
step_name
:
"
FROGS_3
"
,
tool_name
:
"
FROGS_filter_OTU"
,
rule_name
:
"
FROGS_filter_OTU
"
,
{
step_name
:
"
quantification
"
,
tool_name
:
"
htseq_count"
,
rule_name
:
"
htseq_count
"
,
params
:
[
{
input_name
:
sequence_file
,
origin_command
:
FROGS_remove_chimera
,
origin_name
:
non_chimera_fasta
,
origin_step
:
FROGS
},
{
input_name
:
biom
,
origin_command
:
FROGS_remove_chimera
,
origin_name
:
abundance_file
,
origin_step
:
FROGS
},
{
input_name
:
bam
,
origin_command
:
hisat2_SE
,
origin_name
:
bam
,
origin_step
:
mapping
},
{
input_name
:
gff_file
,
origin_command
:
raw_gff3
,
origin_name
:
gff3
,
origin_step
:
input
},
]
},
{
step_name
:
"
FROGS_5"
,
tool_name
:
"
FROGS_affiliation"
,
rule_name
:
"
FROGS_affiliation
"
,
{
step_name
:
"
differential_expression"
,
tool_name
:
"
deseq2"
,
rule_name
:
"
deseq2
"
,
params
:
[
{
input_name
:
fasta
,
origin_command
:
FROGS_filter_OTU
,
origin_name
:
filtered_sequences
,
origin_step
:
FROGS_3
},
{
input_name
:
biom
,
origin_command
:
FROGS_filter_OTU
,
origin_name
:
abundance_file
,
origin_step
:
FROGS_3
},
]
},
{
step_name
:
"
FROGS_6"
,
tool_name
:
"
FROGS_filter_affiliation"
,
rule_name
:
"
FROGS_filter_affiliation"
,
params
:
[
{
input_name
:
fasta
,
origin_command
:
FROGS_filter_OTU
,
origin_name
:
filtered_sequences
,
origin_step
:
FROGS_3
},
{
input_name
:
biom
,
origin_command
:
FROGS_affiliation
,
origin_name
:
abundance_file
,
origin_step
:
FROGS_5
},
]
},
{
step_name
:
"
FROGS_8"
,
tool_name
:
"
FROGS_biom_to_tsv"
,
rule_name
:
"
FROGS_biom_to_tsv"
,
params
:
[
{
input_name
:
biom
,
origin_command
:
FROGS_filter_affiliation
,
origin_name
:
biom_filtered
,
origin_step
:
FROGS_6
},
]
},
{
step_name
:
"
FROGS_7"
,
tool_name
:
"
FROGS_phyloseq_stats"
,
rule_name
:
"
FROGS_phyloseq_stats"
,
params
:
[
{
input_name
:
biom_file
,
origin_command
:
FROGS_filter_affiliation
,
origin_name
:
biom_filtered
,
origin_step
:
FROGS_6
},
{
input_name
:
otu_sequence_file
,
origin_command
:
FROGS_filter_affiliation
,
origin_name
:
sequence_file
,
origin_step
:
FROGS_6
},
{
input_name
:
counts
,
origin_command
:
htseq_count
,
origin_name
:
stats
,
origin_step
:
quantification
},
{
input_name
:
popmap_file
,
origin_command
:
raw_popmap
,
origin_name
:
popmap
,
origin_step
:
input
},
]
},
{
step_name
:
"
metabarcoding"
,
tool_name
:
"
lulu"
,
rule_name
:
"
lulu"
,
params
:
[
{
input_name
:
abundance_file
,
origin_command
:
FROGS_biom_to_tsv
,
origin_name
:
lulu_abundance_file
,
origin_step
:
FROGS_8
},
{
input_name
:
sequence_file
,
origin_command
:
FROGS_filter_affiliation
,
origin_name
:
sequence_file
,
origin_step
:
FROGS_6
},
]
},
],
steps
:
[
{
title
:
"
FROGS_2"
,
name
:
"
FROGS_2"
,
tools
:
[
FROGS_preprocess
],
default
:
"
FROGS_preprocess"
},
{
title
:
"
FROGS_4"
,
name
:
"
FROGS_4"
,
tools
:
[
FROGS_cluster
],
default
:
"
FROGS_cluster"
},
{
title
:
"
FROGS"
,
name
:
"
FROGS"
,
tools
:
[
FROGS_remove_chimera
],
default
:
"
FROGS_remove_chimera"
},
{
title
:
"
FROGS_3"
,
name
:
"
FROGS_3"
,
tools
:
[
FROGS_filter_OTU
],
default
:
"
FROGS_filter_OTU"
},
{
title
:
"
FROGS_5"
,
name
:
"
FROGS_5"
,
tools
:
[
FROGS_affiliation
],
default
:
"
FROGS_affiliation"
},
{
title
:
"
FROGS_6"
,
name
:
"
FROGS_6"
,
tools
:
[
FROGS_filter_affiliation
],
default
:
"
FROGS_filter_affiliation"
},
{
title
:
"
FROGS_8"
,
name
:
"
FROGS_8"
,
tools
:
[
FROGS_biom_to_tsv
],
default
:
"
FROGS_biom_to_tsv"
},
{
title
:
"
FROGS_7"
,
name
:
"
FROGS_7"
,
tools
:
[
FROGS_phyloseq_stats
],
default
:
"
FROGS_phyloseq_stats"
},
{
title
:
"
metabarcoding"
,
name
:
"
metabarcoding"
,
tools
:
[
lulu
],
default
:
"
lulu"
},
{
title
:
"
quality"
,
name
:
"
quality"
,
tools
:
[
trimmomatic
],
default
:
"
trimmomatic"
},
{
title
:
"
quality_2"
,
name
:
"
quality_2"
,
tools
:
[
fastqc
],
default
:
"
fastqc"
},
{
title
:
"
mapping"
,
name
:
"
mapping"
,
tools
:
[
hisat2
],
default
:
"
hisat2"
},
{
title
:
"
quantification"
,
name
:
"
quantification"
,
tools
:
[
htseq_count
],
default
:
"
htseq_count"
},
{
title
:
"
differential_expression"
,
name
:
"
differential_expression"
,
tools
:
[
deseq2
],
default
:
"
deseq2"
},
],
input
:
[
"
raw_read
_dir
"
,
"
raw_popmap"
,],
input
:
[
"
raw_read
s"
,
"
raw_hisat2_index"
,
"
raw_gff3
"
,
"
raw_popmap"
,],
params_equals
:
[
]
...
...
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