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exploitation

exploitation

People who use our scripts to process eDNA metabarcoding NGS data

  • B

    The first step to begin with obitools and Bash

  • D

    This project has for goal to filtrate efficiently eDNA sequences after PCR amplification and NGS, in order to eliminate the false sequences generated by these technologies.

  • E

    This project aims to compare twelve bioinformatics pipelines based on five existing metabarcoding programs to make recommendations for data management in intraspecific variability studies using environmental DNA.

  • O

    This project presents a pipeline based on OBITools commands in order to eliminate sequences interpreted as coming from PCR errors and to assign each remaining sequence to a taxon thanks to a reference database.

  • P

    How to design a primer ?

  • P

    This project permits to check the specificity of already designed primers.

  • P

    Visualization of the specificity of designed primers with mismatch plot

  • S

    This project is based on the idea that gathering similar sequences allows to faithfully study them by eliminating sequences generated from PCR or NGS errors.