... | @@ -237,8 +237,17 @@ The [assembly Snakefile](https://gitlab.mbb.univ-montp2.fr/edna/snakemake_rapidr |
... | @@ -237,8 +237,17 @@ The [assembly Snakefile](https://gitlab.mbb.univ-montp2.fr/edna/snakemake_rapidr |
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* results/02_assembly/02_remove_unaligned/`run`.ali.fastq: aligned and merged sequences fastq file
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* results/02_assembly/02_remove_unaligned/`run`.ali.fastq: aligned and merged sequences fastq file
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#### 3. Demultiplexing
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### 3. Demultiplexing
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#### 3.1. Assign each sequence record to the corresponding `sample`
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[assign_sequences](https://gitlab.mbb.univ-montp2.fr/edna/snakemake_rapidrun_obitools/-/blob/master/03_demultiplex/rules/assign_sequences.smk): assign each sequence record to the corresponding `projet`/`marker`/run`/`plaque` == `sample`
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* inputs:
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* results/02_assembly/02_remove_unaligned/`run`.ali.fastq: aligned and merged sequences fastq file
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* `marker` sample description .dat file
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* output:
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* results/03_demultiplex/01_assign_sequences/`projet`/`marker`/run`.ali.assigned.fastq
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... | @@ -257,10 +266,6 @@ The [assembly Snakefile](https://gitlab.mbb.univ-montp2.fr/edna/snakemake_rapidr |
... | @@ -257,10 +266,6 @@ The [assembly Snakefile](https://gitlab.mbb.univ-montp2.fr/edna/snakemake_rapidr |
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## assemble
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### Paired end alignment then keep reads with quality > 40
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### Remove unaligned sequence records
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## demultiplex
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### Assign each sequence record to the corresponding sample/marker combination
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### Assign each sequence record to the corresponding sample/marker combination
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### Split the input sequence file in a set of subfiles according to the values of attribute `sample`
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### Split the input sequence file in a set of subfiles according to the values of attribute `sample`
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## filter samples
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## filter samples
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