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This workflow will use a pseudo aligner (Kallisto or Salmon) to quantify transcript abundance and test for differential expression.
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Comparison of multiple bioinformatic programs for each step of eDNA analysis
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Codes for the paper : "Genomic resources for Mediterranean fishes"
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remy / website_checks
MIT LicenseSome basic tests to check websites :
https certificates, checksum of the index web page, HTTP answers.Updated -
Seek positions of variants onto genome and check if they are on an annotated region e.g. coding region. Then seek the proteic function of the gene
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remy / BibEthique
Creative Commons Zero v1.0 UniversalThis project is aimed at providing a list of "Ethical' journals where ISEM's researchers are used to publishing there works.
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This project presents a pipeline based on OBITools commands in order to eliminate sequences interpreted as coming from PCR errors and to assign each remaining sequence to a taxon thanks to a reference database.
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This project is based on the idea that gathering similar sequences allows to faithfully study them by eliminating sequences generated from PCR or NGS errors.
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edna / snakemake_rapidrun_swarm
MIT LicenseOTU clustering with SWARM on RAPIDRUN data encapsulated in SNAKEMAKE
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This project has for goal to filtrate efficiently eDNA sequences after PCR amplification and NGS, in order to eliminate the false sequences generated by these technologies.
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Codes Pierre-Edouard Guerin wrote for the scientific paper "Predicting genotype environmental range from genome–environment associations"
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Here is a version of the pipeline "only_obitools" using nextflow, a reactive workflow framework and a programming DSL that eases writing computational pipelines with complex data.
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Data for the tutorial on how to analyze DNA metabarcoding data produced on Illumina sequencers
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Simulations of metabarcoding data from fish 12S Teleo1
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